By John H. Luft (auth.), James K. Koehler Ph. D. (eds.)
The previous decade has noticeable a extraordinary bring up within the use of electron microscopy as a researm instrument in biology and medication. hence, so much institu tions of upper studying now boast numerous electron optical laboratories having quite a few degrees of class. education within the regimen use of elec tron optical gear and interpretation of effects is not any longer constrained to some prestigious facilities. however, temniques used by researm employees within the ultrastructural area became tremendous diversified and complicated. even if loads of relatively first-class volumes of electron microscopic temnique are actually devoted to the elemental components to be had whim permit the beginner to procure an affordable creation to the sector, quite few books were dedicated to a dialogue of extra advert vanced temnical features of the paintings. It used to be with this view that the current quantity was once conceived as a convenient reference for staff already having a few history within the box, as a knowledge resource for these wishing to shift efforts into extra promising temniques, or to be used as a sophisticated direction or seminar consultant. subject material has been mosen rather at the foundation of pertinence to offer researm actions in organic electron microscopy and emphasis has been given these parts whim look destined to drastically extend in precious ness within the close to future.
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3) The polymer should be mechanically stable to radiation. It may be radiation-resistant, but if not, the mass lost during irradiation should not weaken the embedding material enough to displace the tissue elements within the section. Certainly it should not melt under the beam. 4) The density should be low. The specific gravity of the resin should be as low as possible to provide the greatest contrast for the tissue. Mass loss during radiation may contribute to this provided that strength is retained.
Electron Micr. 2, P-10. : S. BREESE, Jr .. New York: Academic Press 1962. : A new method for embedding with epoxy resin at room temperature. J. Electronmicroscopy 14, 275-283 (1965). LANE, B. , EUROPA, D. : Differential staining of ultrathin sections of Epon-embedded tissues for light microscopy. J. Histochem. Cytochem. 13, 579-582 (1965). LEDUC, E. : Ultrastructural cytochemistry. Enzyme and acid hydrolysis of nucleic acids and proteins. J. biophys. biochem. Cytol. 10, 437-455 (1961). Embedding Media - Old and New 33 LEDUC, E.
Mixed Araldite 502 increases only about 10% over the same length of time. The problem with Bpon 812 may be that it contains some of the triglycidyl ether of glycerol along with the major components of the di- and mono-glycidyl ethers of glycerol (LEE and NEVILLE, 1967, pp. 4-66). The trifuctional component should cross-link the forming resin with concomitant rapid increase in viscosity. On the other hand, the keeping qualities of Bpon 812 are very good. One particular batch of Bpon 812 was titrated for its epoxy equivalent upon arrival, and after 3 years of storage at room temperature with occasional opening for use, the epoxy equivalent was within one percent of its original value.
Advanced Techniques in Biological Electron Microscopy by John H. Luft (auth.), James K. Koehler Ph. D. (eds.)
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